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Small proteins can even be synthesized chemically by a relatives of procedures generally known as peptide synthesis, which trust in organic synthesis approaches for example chemical ligation to create peptides in high yield.[17] Chemical synthesis allows for the introduction of non-natural amino acids into polypeptide chains, such as attachment of

An additional applicable procedure is cofractionation in sucrose (or other product) gradients utilizing isopycnic centrifugation.[49] Though this technique does not establish colocalization of a compartment of acknowledged density plus the protein of desire, it does increase the probability, and is a lot more amenable to big-scale reports.If pernic